| Silver wire, teflon, 0.015" bare, 0.019" coated |
A-M Systems Inc |
7875 |
0 |
11627-A Airport Rd Everett WA 98204 ph 206-3531123 |
| MD-2A motion control system |
Arrick Robotics |
MD-2A |
710 |
|
| X-9 linear positioning table |
Arrick Robotics |
X-9 |
410 |
|
| RT-12 rotary positioning table |
Arrick Robotics |
RT-12 |
310 |
|
| Digidata 1200 A/D board |
Axon Instr. |
|
3220.96 |
|
| Axopatch 200B amplifier |
Axon Instr. |
Axopatch 200B |
9200 |
650-571-9400 |
| pCLAMP 7 software |
Axon Instr. |
|
1308.52 |
includes academic discount of 50% |
| Needle (Yale hypodermic) |
Becton Dickinso |
5122 |
0 |
25G5/8 |
| Spinal Needle |
Becton Dickinso |
405180 |
0 |
25G x 3.5"; 25 needles/pk. |
| Refrigerator (20.6 cu ft) |
Best Buy |
CTX216AX |
587.99 |
|
| Freezer |
Best Buy |
|
429.99 |
NOT frost free |
| Microwave (Sharp) |
Best Buy |
|
71.6 |
|
| Isoflurane (Forane) - 100 ml |
Butler Vet |
AB526000 |
22.67 |
100 ml; Madison, WI 53713 |
| Chart recorder blue long pen |
Cole Parmer |
H-08479-31 |
13 |
pk. of 6 |
| Perfusion pump silicon tubing |
Cole Parmer |
H-96410-13 |
27 |
Platinum-cured size 13 |
| Perfusion pump mounting hardware |
Cole Parmer |
H-07013-05 |
13 |
|
| Perfusion pump head |
Cole Parmer |
H-07013-20 |
70 |
|
| Perfusion pump |
Cole Parmer |
H-07553-50 |
350 |
|
| Fiberoptic lamp light stand |
Cole Parmer |
H-09743-60 |
100 |
|
| Fiberoptic lamp illuminator adapter |
Cole Parmer |
H-09741-20 |
15 |
|
| Fiberoptic lamp illuminator |
Cole Parmer |
H-09741-50 |
295 |
|
| Chart recorder black short pen |
Cole Parmer |
H-08479-34 |
13 |
pk. of 6 |
| Fiberoptic lamp dual gooseneck light pipe |
Cole Parmer |
H-09742-20 |
149 |
|
| Chart recorder paper |
Cole Parmer |
E-08386-45 |
55.5 |
pk. of 3 of 200 mm paper |
| Chart recorder |
Cole Parmer |
E-08376-30 |
1495 |
|
| Fiberoptic lamp replacement bulb |
Cole Parmer |
H-09741-03 |
23 |
150 W bulb; Ushio halogen projector bulb |
| Electrodes recording; 1.2mm OD x 0.9mm ID x 75 mm |
FHC |
30-31-0-75 |
0 |
with omega dot fiber; 250/pk; ph.800-326-2905 |
| Beaker (1 L) |
Fisher |
02-540P |
48 |
pk. of 6 (Corning # 1000-1L) |
| Centrifuge tubes (50 ml Fisherbrand) |
Fisher |
05-539-7 |
107.79 |
case of 500; polypropylene sterile |
| Centrifuge tubes (15 ml Fisherbrand) |
Fisher |
05-539-2 |
141 |
case of 500; sterile |
| Centrifuge tube rack (holds 50 ml tubes) |
Fisher |
14-791-6B |
20 |
holds 16 tubes |
| Cart (Plastic) |
Fisher |
11-926-85 |
146 |
|
| Vials (carrier) |
Fisher |
03-337-27 |
40 |
case of 100 |
| Carboy (polypropylene 20 L with spigot) |
Fisher |
02-963-2B |
91 |
Nalge # 2319-0050 |
| Bottles glass (Wheaton "900" amber 1L) |
Fisher |
02-911-141 |
45 |
case of 12; have TFE liner caps |
| Beaker (2 L) |
Fisher |
02-540R |
63 |
pk. of 4 (Corning # 1000-2L) |
| Balance (Mettler PM600) |
Fisher |
01-911-172 |
1995 |
|
| Balance (Mettler AB54) |
Fisher |
01-913-503A |
1995 |
|
| Dessicator (Nalgene plastic) |
Fisher |
08-642-15 |
28 |
|
| Flask Pyrex 125 ml |
Fisher |
10-040D |
40 |
pk of 12; Corning # 4980-125 |
| Beaker (250 ml) |
Fisher |
02-540K |
34 |
pk. of 12 (Corning # 1000-250) |
| Microcentrifuge (MiniMax) |
Fisher |
05-102-2 |
750 |
|
| Pens - Secureline Biotech Marker |
Fisher |
14-905-23 |
19 |
pack of 10 extrafine pens |
| Pens - Fisherbrand |
Fisher |
13-381 |
15 |
pack of 12 (black) |
| Parafilm (4" x 50') |
Fisher |
13-374-12 |
33 |
|
| Parafilm (2" x 50') |
Fisher |
13-374-16 |
20 |
|
| Microtube storage racks (Fisherbrand) |
Fisher |
05-541-1 |
28 |
pack of 5 |
| Microtube storage box |
Fisher |
15-350-60 |
31 |
pack of 4; for 1.5 ml tubes |
| Microtube storage box |
Fisher |
15-350-59 |
31 |
pack of 4; for 0.5 ml tubes |
| Microtube racks |
Fisher |
14-809-166 |
17 |
pack of 4; float in water bath |
| Microscope setup for recording |
Fisher |
BL-31-26-40-73 |
1713 |
|
| Microscope setup for isolation |
Fisher |
BL-31-26-29-73 |
1345 |
|
| Microscope Nicholas illuminator replacement bulb |
Fisher |
BL-31-31-75 |
17 |
|
| Microscope Nicholas illuminator |
Fisher |
BL-31-33-05-28 |
183 |
|
| Filter System (Corning 200 ml) |
Fisher |
09-761-1 |
43.5 |
case of 12 (Corning # 25932-200) |
| Microdispenser (Drummond 10 ul) |
Fisher |
21-169-20A |
176 |
Drummond # 3-000-510 - micropipetter |
| Incubator (dry bath) |
Fisher |
11-718 |
200 |
|
| Microcentrifuge (Eppendorf 5415C) |
Fisher |
05-400-10 |
2195 |
|
| Methanol |
Fisher |
A412-500 |
11 |
500 ml |
| Kimwipes |
Fisher |
06-666A |
145 |
cs of 60 |
| Isolation dishes (Corning 60x15 mm, top & bottom) |
Fisher |
08-747A |
62 |
pk of 12, Corning # 3160-60 |
| Isolation dishes (Corning 100X15 mm, top & bottom) |
Fisher |
08-747C |
51 |
pk of 12, Corning # 3160-101 |
| Ice buckets |
Fisher |
11-676 |
56 |
that's for black, red is 11-676-36 |
| Graduated cylinder (250 ml) |
Fisher |
08-552-4D |
27 |
(Corning # 3024-250) |
| Funnel, glass, 100 mm diameter |
Fisher |
10-325E |
33.12 |
pk. of 6 |
| Graduated cylinder (100 ml) |
Fisher |
08-552-4C |
20 |
(Corning # 3024-100) |
| Flask Pyrex 250 ml |
Fisher |
10-040F |
41 |
pk of 12; Corning # 4980-250 |
| Filter System (Corning 500 ml) |
Fisher |
09-761-5 |
106 |
case of 12 (Corning # 25942-500) |
| Incubator heating block (dry bath) |
Fisher |
11-718-9 |
60 |
for 1.5 ml tubes |
| Microscope "S" arm |
Fisher |
BL-31-27-14 |
422 |
|
| Glass disposal box (floor model) |
Fisher |
120097A |
43.99 |
pk. of 6 |
| Flask (for vacuum, 2000 ml) |
Fisher |
10180G |
44.57 |
|
| Tubing (Tygon, 1/4 x 1/16 - 10 ft) |
Fisher |
141693C |
6.94 |
|
| Drierite (one pound) |
Fisher |
075783A |
7.16 |
|
| Bunsen burner |
Fisher |
03917 |
14.53 |
|
| Funnel (glass) |
Fisher |
03865 |
3.25 |
|
| Pail (plastic, for oocyte rig drainage) |
Fisher |
133084 |
13.29 |
|
| Brush to clean weigh scale |
Fisher |
03661 |
9.68 |
|
| pH buffers (4, 7 and 10) |
Fisher |
SB105 |
15.26 |
|
| Tape (rainbow pack) |
Fisher |
118805R |
45.47 |
pk. of 16 |
| Tape dispenser (for multicoloured tapes) |
Fisher |
118654 |
17.92 |
|
| Glasswashing brush |
Fisher |
03565 |
4.54 |
|
| Sharps container |
Fisher |
1482763 |
80.69 |
one gallon capacity |
| Flask Pyrex 25 ml |
Fisher |
10040B |
26.22 |
pk. of 12 |
| Pippetter Eppendorf series 2000 (50-200 ul) |
Fisher |
05-402-49 |
215.28 |
|
| Isopropanol (2-propanol) |
Fisher |
A4161 |
10.81 |
1 L |
| Hydrochloric acid (12N) |
Fisher |
A144500 |
9.47 |
500 ml |
| Safety glasses (UV) |
Fisher |
17286 |
12.66 |
|
| Rubber bulbs |
Fisher |
14065A |
19.49 |
pk. of 72 |
| Tris base |
Fisher |
152500 |
30.23 |
500 g |
| Ethidium bromide |
Fisher |
130210 |
23.48 |
10 ml solution |
| Agarose (high melting) |
Fisher |
164100 |
123.22 |
100 g |
| Racks (foam) for 30 tubes |
Fisher |
0566415D |
19 |
6 per pack |
| Glove for autoclave |
Fisher |
11394299 |
12.81 |
|
| Gloves latex - large |
Fisher |
113944C |
13.56 |
pack of 100 |
| Gloves latex -small |
Fisher |
113944A |
13.56 |
pack of 100 |
| Flask Pyrex 50 ml |
Fisher |
10040C |
26.22 |
pk. of 12 |
| Media bottles (Pyrex) 1000 ml |
Fisher |
06-414-1D |
56.7 |
10/case |
| Wash bottle (500 ml) |
Fisher |
0340910E |
10.99 |
4 per pack |
| Test tube support (17-20 mm) |
Fisher |
1478117 |
11 |
|
| Graduated cylinder (1000 ml) |
Fisher |
08-552-4F |
27.28 |
(Corning # 3024-1L) |
| Bench lab cover (20" x 300') |
Fisher |
1420630 |
98.04 |
case of 2 |
| Falcon tubes (#2059) |
Fisher |
14-959-11B |
83.22 |
case of 500 as 25/pk |
| pipetter tips 0.5-10 ul (aerosol-resistant) |
Fisher |
21-236-5 |
382.5 |
case |
| Microscope "A" Arm (for oocyte isolation) |
Fisher |
BL-31-26-88-87 |
270 |
|
| Scalpel blades #11 |
Fisher |
08-916-5B |
42.89 |
100/pk. |
| Scalpel handle |
Fisher |
08-913-5 |
12.5 |
|
| acetic acid (glacial) |
Fisher |
A35-500 |
11.69 |
500 ml |
| Centrifuge tubes Nalgene 30 ml polypropylene |
Fisher |
05-563-10E |
40.85 |
10/pk |
| Stoppers (rubber #9 with one hole) |
Fisher |
14135N |
12.3 |
|
| Microcentrifuge tubes (0.5 ml, Fisherbrand) |
Fisher |
05-406-15 |
12.98 |
1000/pk |
| Vials scintillation (20 ml, with cap) |
Fisher |
033374 |
61.2 |
case of 500 |
| Media bottles (Pyrex) 500 ml |
Fisher |
06-414-1C |
46.2 |
10/case |
| Media bottles (Pyrex) 250 ml |
Fisher |
06-414-1B |
38.58 |
10/case |
| Media bottles (Pyrex) 100 ml |
Fisher |
06-414-1A |
34.8 |
10/case |
| Syringes B-D brand disposable 10 cc |
Fisher |
14-823-2A |
16.93 |
100/pk. |
| Pipettes Fisherbrand 50 ml (individually wrapped) |
Fisher |
13-676-10R |
119.73 |
100/pk. |
| Pipet-Aid replacement filters |
Fisher |
13-681-18C |
13.3 |
5/pk. |
| pipette sterilizing box (metal) |
Fisher |
03-465 |
19.02 |
|
| innoculating turntable |
Fisher |
08-758 |
80.3 |
|
| innoculating loops |
Fisher |
DF1906-96-8 |
25.69 |
250/pk |
| Petri dishes (Fisherbrand) 100 x 15 mm |
Fisher |
08-757-12 |
44.28 |
500/case |
| Cell spreaders (Fisherbrand) |
Fisher |
05-541-11 |
14.64 |
25/pk |
| Pipet station (Fisherbrand) |
Fisher |
06-666-56 |
40 |
|
| Microcentrifuge tubes (1.5 ml, Fisherbrand) |
Fisher |
05-406-16 |
11 |
1000/pk |
| Biohazard bag large rack |
Fisher |
01-815-9C |
60.03 |
|
| Water bath (2L) |
Fisher |
15-461-2S |
560 |
|
| Weighing dishes Fisherbrand large hexagonal |
Fisher |
02-202D |
113 |
500/pk |
| Weighing paper Fisherbrand low nitrogen |
Fisher |
09-898-12A |
14 |
500/pk |
| Well plates (96 wells) |
Fisher |
07-200-90 |
64 |
case of 50 |
| pH meter (Accumet Basic) |
Fisher |
13-635-BAS |
700 |
|
| pipetter tips (blue; 201-1000 ul) |
Fisher |
21-197-8A |
45 |
10 racks of 100 each |
| scrubbing brush (small) |
Fisher |
03-608 |
11.22 |
pk/12 |
| Flask Pyrex 500 ml |
Fisher |
10-040H |
26 |
pk of 6; Corning # 4980-500 |
| Beakers plastic (set) |
Fisher |
02-591-16 |
13.62 |
|
| Biohazard bag small rack |
Fisher |
01-815-10A |
26.99 |
|
| Vortex mixer |
Fisher |
12-814 |
183 |
|
| DTT |
Fisher |
BP172-5 |
53.67 |
|
| autoclave tape |
Fisher |
11-875-52 |
3.04 |
1" wide x 500" |
| Biohazard bags (orange) |
Fisher |
01-815-C |
55.56 |
cs. Of 100 |
| Weighing dishes Fisherbrand small hexagonal |
Fisher |
02-202A |
23.51 |
cs. Of 500 |
| Pippetter Oxford 1-5 ml |
Fisher |
21-251 |
216 |
|
| Stir bars (small 1/2") |
Fisher |
14-511-61 |
2.45 |
|
| 1000-5000 ul pipet tips |
Fisher |
211987D |
13.49 |
bag of 250 |
| Microscope |
Fisher |
BL312694 |
780.48 |
just the microscope |
| Steriflip filter |
Fisher |
SCGP00525 |
82.8 |
25/pk, for 50 ml centrifuge tubes |
| Microscope 10X eyepiece |
Fisher |
BL31158102 |
156 |
|
| pipetter tips (1-5 ml) |
Fisher |
21-197-8D |
13.49 |
pk. Of 250 (bulk) |
| pipetter tips (1-200 µl) aerosol-resistent |
Fisher |
21-236-1 |
76.5 |
10 trays of 96 |
| Bottles, clear glass 2L (pk. of 6) |
Fisher |
02-911-92 |
30.59 |
|
| step stool |
Fisher |
11-931-1 |
32.1 |
|
| Sodium hydroxide 10N solution |
Fisher |
SS-255-1 |
26 |
1L |
| pipetter tips (clear; 0.5 - 10 ul) |
Fisher |
21-197-2F |
43 |
bulk pack of 1000 |
| pipetter tips (yellow; 1-200 ul) |
Fisher |
21-197-8E |
45 |
10 racks of 96 each |
| pipetter tips for positive displacement |
Fisher |
21-379-72 |
126 |
5 racks of 96 |
| Pipet-Aid |
Fisher |
13-681-19 |
245 |
|
| pipetter (Brinkmann 0.5-10 ul) |
Fisher |
21-381-200 |
300 |
Brinkmann # 22 44 000-4 |
| Stir bars Fisherbrand octagonal |
Fisher |
14-511-64 |
4 |
1.5" long |
| Spatula Fisher spoonula |
Fisher |
14-357-10 |
31 |
pk. of 6 |
| Spatula (teflon coated, for small amounts) |
Fisher |
21-401-18 |
8 |
|
| pipetter tips (clear; 0.5 - 10 ul) |
Fisher |
21-197-2E |
54 |
10 racks of 96 each |
| pipetter tips (blue; 201-1000 ul) |
Fisher |
21-197-8F |
38 |
Bulk pack of 1000 |
| Scoop (polyethylene) |
Fisher |
14-236-1 |
6 |
for handling/anaesthetizing frogs |
| pipettes (microdispenser injection 10 ul) |
Fisher |
21-169A |
11 |
pk of 100 Drummond # 510G-310G-210G |
| Timer (triple display) |
Fisher |
06-662-3 |
20 |
|
| Flask volumetric 2L |
Fisher |
10-210H |
60 |
Corning # 5640-2L |
| Tray (Stainless-steel for frog surgery) |
Fisher |
13-361C |
30 |
|
| Spatula Fisher scoopula |
Fisher |
14-357 |
10 |
pk. of 6 |
| Transfer pipettes (standard) |
Fisher |
13-711-7 |
26 |
pack of 500 |
| pipettes (10 ml glass Fisherbrand) |
Fisher |
13-678-31J |
147 |
case of 500 (non-sterile, non-plugged) |
| Stirrer |
Fisher |
11-500-7S |
185 |
49 sq. inch ceramic top surface |
| pipetter (Brinkmann 10-100 ul) |
Fisher |
21-381-202 |
300 |
Brinkmann # 22 44 010-1 |
| pipetter (Brinkmann 100-1000 ul) |
Fisher |
21-381-204 |
300 |
Brinkmann # 22 44 020-9 |
| autoclave tape |
Fisher |
11-880-30A |
1.43 |
1/2" wide x 500" |
| pipetter (Brinkmann positive displacement 1-20 ul) |
Fisher |
21-379-71 |
300 |
Brinkmann # 21-379-71 |
| Power supply Model 250 |
Gibco BRL |
11066-016 |
365 |
|
| Gel electrophoresis appar.Sunrise 12-16 horizontal |
Gibco BRL |
11068-129 |
305 |
|
| gel electrophoresis apparatus Horizon 58 horizont. |
Gibco BRL |
41060-013 |
240 |
|
| 1 kb DNA ladder |
Gibco BRL |
15615-016 |
105 |
250 ug |
| Luria Broth base |
Gibco BRL |
12795-027 |
19.3 |
500 g |
| Luria agar |
Gibco BRL |
12945-036 |
43.6 |
500 g |
| SOC medium |
Gibco BRL |
15544-018 |
48 |
10 vials of 10 ml |
| SNAP miniprep kit |
Invitrogen |
K1900-05 |
740 |
|
| Suture Silk 6-0, G-1, 18" |
McKesson Medica |
002746 |
62 |
10 per box |
| Enflurane (Ethrane) |
McKesson Medica |
305032 |
83.01 |
250 ml; Madison, WI 53713 |
| QIAfilter Plasmid Maxi Kit (10) |
Qiagen |
12262 |
|
|
| FG-7142 |
RBI |
E-006 |
68 |
100 mg |
| Alphaxalone |
RBI |
A-175 |
35 |
5 mg |
| Propofol (2,6-diisopropyl phenol) |
RBI |
P-185 |
17 |
5 g |
| RO 15-4513 |
RBI |
R-109 |
49 |
25 mg |
| DMCM |
RBI |
E-007 |
46 |
25 mg |
| Halothane |
RBI |
H-169 |
67 |
250 ml |
| Serotonin |
RBI |
S-011 |
37 |
100 mg as hydrochloride |
| Scissors for cutting injection pipetter tips |
Roboz |
RS-5610 |
214 |
ph. 1-800-424-2984 |
| Forceps (hemostatic, for frog surgery) |
Roboz |
RS-7231 |
77 |
ph. 1-800-424-2984 |
| Tweezers #55 |
Roboz |
RS-5010 |
27 |
ph. 1-800-424-2984 |
| Forceps (microdissecting, for frog surgery) |
Roboz |
RS-5139 |
32 |
ph. 1-800-424-2984 |
| lauryl sulfate (SDS) |
Sigma |
L5750 |
11.8 |
100 g |
| Sodium acetate |
Sigma |
S2889 |
14.5 |
250 g |
| 1,1,1-trichloroethane |
Sigma |
T4678 |
19.05 |
500 ml |
| Kanamycin solution |
Sigma |
K0254 |
25.4 |
20 ml of 50 mg/ml solution |
| glucose |
Sigma |
G5400 |
7.5 |
250 g |
| Sucrose |
Sigma |
S0389 |
19.9 |
500 g |
| Hexanol |
Sigma |
H-4504 |
7.45 |
|
| Pentanol |
Sigma |
P-5709 |
19.75 |
|
| Butanol (n-butanol) |
Sigma |
B-1417 |
17.75 |
|
| Propanol (n-propanol) |
Sigma |
P-6334 |
21.85 |
|
| TEMED |
Sigma |
T7024 |
18.6 |
25 ml |
| Heptanol |
Sigma |
H-6129 |
7.4 |
|
| Nonanol |
Sigma |
N-1754 |
10.35 |
|
| methyl ethyl ketone |
Sigma |
M2886 |
13.15 |
500 ml |
| Alprazolam |
Sigma |
A-8800 |
22.6 |
|
| Clonazepam |
Sigma |
C-1277 |
27.4 |
|
| Zinc chloride |
Sigma |
Z-4875 |
9.6 |
|
| Extraction filter for ethidium bromide |
Sigma |
Z36,156-9 |
31.5 |
2/pk. |
| Tetracycline |
Sigma |
T-8032 |
7.25 |
|
| methanol |
Sigma |
M3641 |
10.9 |
500 ml |
| Ampicillin |
Sigma |
A-2804 |
9.1 |
|
| EDTA |
Sigma |
ED4SS |
23.95 |
|
| Octanol |
Sigma |
O-4500 |
5.8 |
|
| Toluene |
Sigma |
T4428 |
10.65 |
500 ml |
| Tridecanol |
Sigma |
T-6757 |
21.2 |
|
| Penicillin-streptomycin solution |
Sigma |
P-0781 |
6.4 |
|
| Gentamicin solution |
Sigma |
G-1397 |
36.75 |
|
| Magnesium chloride hexahydrate |
Sigma |
M-2670 |
16.2 |
|
| Theophylline |
Sigma |
T-1633 |
14.35 |
|
| Undecanol |
Sigma |
U-4376 |
6.95 |
|
| Dodecanol (lauryl alcohol) |
Sigma |
L-5375 |
6.75 |
|
| acetone |
Sigma |
A4206 |
10.75 |
500 ml |
| Trichloroethylene |
Sigma |
T4928 |
17.65 |
500 ml |
| Decanol |
Sigma |
D-0634 |
6.25 |
|
| Xylene |
Sigma |
X5750 |
52.25 |
5g |
| Sodium Chloride |
Sigma |
S-9625 |
44 |
5 kg |
| HEPES |
Sigma |
H-3375 |
163 |
500 g |
| Magnesium sulfate (heptahydrate) |
Sigma |
M-9397 |
24 |
500 g |
| Mineral oil |
Sigma |
M-5904 |
19 |
500 ml |
| Pentobarbital |
Sigma |
P-3761 |
36.35 |
25 g as sodium salt |
| Phenobarbital |
Sigma |
P-5178 |
10 |
5 g as sodium salt |
| Picrotoxin |
Sigma |
P-1675 |
12.2 |
5 g |
| Glycine |
Sigma |
G-7403 |
16 |
100 g |
| Pyruvic acid |
Sigma |
P-2256 |
48.75 |
100 g; sodium salt |
| GABA |
Sigma |
A-5835 |
20 |
25 g |
| phenol:chloroform: isoamyl alcohol (25:24:1) |
Sigma |
P3803 |
31.45 |
100 ml |
| Sodium bicarbonate |
Sigma |
S-6297 |
15 |
250 g |
| DEPC |
Sigma |
D5758 |
16.8 |
5 ml |
| Strychnine hydrochloride |
Sigma |
S-8753 |
9.7 |
25 g |
| Tricaine anaesthetic |
Sigma |
A-5040 |
114.35 |
100 g 3-aminobenzoic acid ethyl ester |
| Zolpidem |
Sigma |
Z-1128 |
24.4 |
50 mg as hemitartrate salt |
| Potassium Chloride |
Sigma |
P-4504 |
14 |
500 g |
| Calcium Nitrate |
Sigma |
C-1396 |
25 |
500 g |
| chloroform |
Sigma |
C2432 |
24.3 |
500 ml |
| phenol |
Sigma |
P1037 |
38.85 |
100 g |
| potassium acetate |
Sigma |
P1190 |
14.9 |
100 g |
| glycerol |
Sigma |
G5516 |
22.4 |
100 ml |
| mercaptoethanol (beta) |
Sigma |
M3148 |
7.4 |
25 ml |
| Trizma acetate |
Sigma |
T1258 |
47.75 |
100 g |
| Calcium Chloride |
Sigma |
C-5080 |
32 |
500 g |
| Flunitrazepam |
Sigma |
F-9261 |
27.45 |
100 mg |
| Collagenase |
Sigma |
C-9891 |
138 |
1 g |
| DMSO (dimethyl sulfoxide) |
Sigma |
D-5879 |
23 |
500 ml |
| Diazepam |
Sigma |
D-0899 |
29.5 |
100 mg |
| Bicuculline methiodide |
Sigma |
B-6889 |
57.65 |
100 mg |
| Micromanipulator stand on magnetic base |
Stoelting |
55110 |
175 |
|
| Micromanipulator MM33 (left hand) |
Stoelting |
55233 |
740 |
|
| Micromanipulator MM33 (right hand) |
Stoelting |
55133 |
740 |
|
| JM109 E. coli host strain |
Stratagene |
200271 |
70 |
0.5 ml glycerol stock |
| Stratacooler |
Stratagene |
400002 |
185 |
for blue (400008 for red) |
| Quikchange Mutagenesis Kit |
Stratagene |
|